资源类型

期刊论文 415

会议视频 6

年份

2024 1

2023 25

2022 38

2021 42

2020 25

2019 42

2018 24

2017 29

2016 18

2015 20

2014 18

2013 25

2012 11

2011 22

2010 11

2009 21

2008 10

2007 14

2006 2

2005 2

展开 ︾

关键词

燃料电池 7

固体氧化物燃料电池 6

干细胞 3

SOFC 2

临床试验 2

催化剂 2

双极板 2

固体氧化物电解池 2

太阳电池 2

嵌合抗原受体 2

氢燃料电池 2

氢能 2

组织工程 2

高压 2

2035 1

3D支架平台 1

3D细胞容器 1

6G；广域覆盖信令小区；多维资源分配；深度Q网络（DQN） 1

BPA 1

展开 ︾

检索范围：

排序： 展示方式：

Using mRNA to investigate the effect of low-pressure ultraviolet disinfection on the viability of

Chao Yang, Wenjun Sun, Xiuwei Ao

《环境科学与工程前沿（英文）》 2019年 第13卷 第2期 doi: 10.1007/s11783-019-1111-x

摘要：

UV can induce damages on mRNA consistently among different genes.

SOS response was more active after UV treatment.

Programmed cell death was not found to be more active after UV treatment.

关键词： UV disinfection     Viability     mRNA     SOS response     Programmed cell death    

HTML PDF 收藏

Orlistat induces ferroptosis-like cell death of lung cancer cells

《医学前沿（英文）》 2021年 第15卷 第6期   页码 922-932 doi: 10.1007/s11684-020-0804-7

摘要： Aberrant de novo lipid synthesis is involved in the progression and treatment resistance of many types of cancers, including lung cancer; however, targeting the lipogenetic pathways for cancer therapy remains an unmet clinical need. In this study, we tested the anticancer activity of orlistat, an FDA-approved anti-obesity drug, in human and mouse cancer cells in vitro and in vivo, and we found that orlistat, as a single agent, inhibited the proliferation and viabilities of lung cancer cells and induced ferroptosis-like cell death in vitro. Mechanistically, we found that orlistat reduced the expression of GPX4, a central ferroptosis regulator, and induced lipid peroxidation. In addition, we systemically analyzed the genome-wide gene expression changes affected by orlistat treatment using RNA-seq and identified FAF2, a molecule regulating the lipid droplet homeostasis, as a novel target of orlistat. Moreover, in a mouse xenograft model, orlistat significantly inhibited tumor growth and reduced the tumor volumes compared with vehicle control (P<0.05). Our study showed a novel mechanism of the anticancer activity of orlistat and provided the rationale for repurposing this drug for the treatment of lung cancer and other types of cancer.

关键词： orlistat     ferroptosis     FAF2     lung cancer    

HTML PDF 收藏

Immunological effects of nano-enabled hyperthermia for solid tumors: opportunity and challenge

《化学科学与工程前沿（英文）》 2022年 第16卷 第3期   页码 333-344 doi: 10.1007/s11705-021-2059-5

摘要： Compared to conventional hyperthermia that is limited by low selectivity and severe side effects, nano-enabled hyperthermia yields great potentials to tackle these limitations for cancer treatment. Another major advance is the observation of immunological responses associated with nano-enabled hyperthermia, which introduces a new avenue, allowing a potential paradigm shift from the acutely effective and cytotoxicity-centric response to the next-phase discovery, i.e., long-lasting and/or systemic anti-tumor immunity. This perspective first discusses the temperature-gradient and the spatially-structured immunological landscape in solid tumors receiving nano-enabled hyperthermia. This includes the discussion about underlying mechanism such as immunogenic cell death, which initiates a profound immunological chain reaction. In order to propagate the immune activation as a viable therapeutic principle, we further discussed the tumor type-specific complexity in the immunological tumor microenvironment, including the creative design of nano-enabled combination therapy to synergize with nano-enabled hyperthermia.

关键词： nano-enabled hyperthermia     immunogenic cell death     heterogeneous immunological landscape     tumor microenvironment    

HTML PDF 收藏

Outcomes of patients awaiting lung transplantation after the implementation of donation after brain death

《医学前沿（英文）》 2022年 第16卷 第5期   页码 760-765 doi: 10.1007/s11684-021-0899-5

摘要： Voluntary contribution has become the only source of donor lungs in China since 2015. To elaborate the outcomes of patients awaiting lung transplantation (LTx) after the implementation of donation after brain death, we performed a retrospective study that encompassed 205 patients with end-stage lung disease who registered for LTx at Shanghai Pulmonary Hospital from January 1, 2015 to January 1, 2021. A total of 180 patients were enrolled in the study. The median waiting time was 1.25 months. Interstitial lung disease (ILD) (103/180, 57.2%) and chronic obstructive pulmonary disease (COPD) (56/180, 31.1%) were the most common diseases in our study population. The mean pulmonary artery pressure (mPAP) of patients in the died-waiting group was higher than that of the survivors (53.29±21.71 mmHg vs. 42.11±18.58 mmHg, P=0.002). The mortality of patients with ILD (34/103, 33.00%) was nearly twice that of patients with COPD (10/56, 17.86%) while awaiting LTx (P=0.041). In the died-waiting group, patients with ILD had a shorter median waiting time than patients with COPD after being listed (0.865 months vs. 4.720 months, P=0.030). ILD as primary disease and mPAP > 35 mmHg were two significant independent risk factors for waitlist mortality, with hazard ratios (HR) of 3.483 (95% CI 1.311–9.111; P=0.011) and 3.500 (95% CI 1.435–8.536; P=0.006). Hence, LTx is more urgently needed in patients with ILD and pulmonary hypertension.

关键词： lung transplantation     donation after brain death     waitlist    

HTML PDF 收藏

The influence of brain death on donor liver and the potential mechanisms of protective intervention

null

《医学前沿（英文）》 2011年 第5卷 第1期   页码 8-14 doi: 10.1007/s11684-011-0109-y

摘要：

Brain-dead donors have become one of the main sources of organs for transplantation in Western countries. The quality of donor organs is closely related to the outcome of the transplantation. Experimental studies have confirmed the inferior graft survival of livers from brain-dead donors compared with those from living donors. Studies conducted in the past 10 years have shown that brain death is associated with effects on the decreased donor organ quality. However, whether the decrease in the viability of donor organs is caused by brain death or by the events before and after brain death remains uncertain. The purpose of this review is to introduce the advances and controversies regarding the influence of brain death on the viability of donor livers and to summarize the mechanisms of the different protective interventions for donor livers.

关键词： brain death     donor liver    

HTML PDF 收藏

Experimental study on the establishment and maintenance of brain death model with pigs

ZHANG Shuijun, SHI Jihua, ZHAI Wenlong, SONG Yan, CHEN Shi

《医学前沿（英文）》 2007年 第1卷 第2期   页码 161-166 doi: 10.1007/s11684-007-0030-6

摘要： It remains controversial that after the transplantation of using grafts from brain-dead donors, organs injury and rejection can influence the effects of transplantation. This study sought to explore methods of establishing a stable brain death (BD) model using Bama mini pigs and to maintain the brain-dead state for a comparatively long period to provide a model for investigating changes in brain death. Sixteen anesthetized Bama mini pigs were randomized into a control group ( = 5) and a BD group ( = 11). Intracranial pressure (ICP) was increased in a modified, slow, and intermittent way to establish BD. Respiration and circulation were sustained during the brain-dead state. Hemodynamic changes were monitored during the experiment. In the BD group, 10 pigs met the requirements for brain death and 1 died of cardiopulmonary complications following an increase in ICP. Brain death was maintained for more than 48 hours with artificial life support. During the experiment, the heart rate and blood pressure showed characteristic changes due to increased ICP. Prior to BD being established, a tic reaction inevitably occurred. We used an improved method of increasing ICP to establish a stable BD model. The BD state could be maintained for more than 48 hours with effective respiratory and circulatory support. Disappearance of the tic reaction was considered to be one of the verified indexes for BD via encephalic pressure increase.

关键词： BD     control     Hemodynamic     cardiopulmonary     modified    

HTML PDF 收藏

Sudden death due to arrhythmogenic right ventricular cardiomyopathy: Two case reports

CHEN Xinshan, ZHANG Yigu, RAO Guangxun, HUANG Guangzhao

《医学前沿（英文）》 2007年 第1卷 第3期   页码 338-342 doi: 10.1007/s11684-007-0065-8

摘要： Arrhythmogenic right ventricular cardiomyopathy (ARVC) is a kind of primary myocardial disease characterized by the regional or global replacement of right ventricular myocardium by fatty and fibrolipomatous tissues. The ARVC, usually presenting with different clinical manifestations and pathological changes, were mainly seen in young men and is one of the main causes of sudden death in the young. Here two autopsied cases of Chinese men aged 30 and 23 years old who appeared healthy but died suddenly while at work are reported respectively. One of the victims had extensive and severe pathological changes in his heart involving the left ventricular wall as well as the ventricular septum and the right atrium. Not only was there a global fatty and fibrolipomatous tissue replacement of the right ventricular myocardia, but also mild sarcoplasmic coagulation in the myocardium and focal lymphocytic infiltration in the myocardial interstitium of the right ventricular wall. In addition, slight atherosclerosis of the coronary artery and intimal thickening of the sino-atrial node were observed. It is believed that there are no marked differences in the pathological changes of ARVC between Chinese patients and patients from western countries. The etiology and pathogenesis of ARVC could not be explained by a single cause or factor and they are probably related to various congenital and acquired causes or factors.

关键词： sarcoplasmic coagulation     acquired     ventricular myocardium     sino-atrial     autopsied    

HTML PDF 收藏

Protein aggregation in association with delayed neuronal death in rat model of brain ischemia

GE Pengfei, FU Shuanglin, LI Wenchen, WANG Chonghao, ZHOU Chuibing, LUO Yinan, LUO Tianfei

《医学前沿（英文）》 2008年 第2卷 第1期   页码 70-74 doi: 10.1007/s11684-008-0013-2

摘要： To investigate the relationship between protein aggregation and delayed neuronal death, we adopted rat models of 20 min ischemia. Brain ischemia was produced using the 2-vessel occlusion (2VO) model in rats Light microscopy, transmission electronic microscopy and Western blot analysis were performed for morphological analysis of neurons, and protein detection. The results showed delayed neuronal death took place at 72 h after ischemia-reperfusion, protein aggregates formed at 4 h after reperfusion and reached the peak at 24 h after reperfusion, and Western blot analysis was consistent with transmission electronic microscopy. We conclude that protein aggregation is one of the important factors leading to delayed neuronal death.
HTML PDF 收藏

Assessing the premature death due to ambient particulate matter in China’s urban areas from 2004 to 2013

Guoxia MA, Jinnan WANG, Fang YU, Xiaomin GUO, Yanshen ZHANG, Chao LI

《环境科学与工程前沿（英文）》 2016年 第10卷 第5期 doi: 10.1007/s11783-016-0849-7

摘要： The number of annual premature deaths attributable to China’s air pollution ranged from 350000 to 520000 from 2004 to 2013, while the figure in 2013 reached 9.9% of total deaths in China. In 2004, the life expectancy potential years of life lost was 69.6 and 1.85 years respectively in contrast to 74.4 and 0.67 years respectively in 2013. The number of PYLL attributable to air pollution in the northern regions is found to be larger than that of the southern regions. The Environmental Burden of Disease (EBD) approach for outdoor air pollution has been used to calculate premature deaths and average potential years of life lost attributable to air pollution in China over the past 10 years with differences between the North and the South of the country being analyzed. The results indicate that: (1) Between 2004 and 2013, annual premature deaths attributable to outdoor air pollution in China ranged from 350000 to 520000. In 2013, deaths resulting from air pollution in China represented 9.9% of the country’s total deaths. (2) In 2004, the average life expectancy of the Chinese population and the number of potential years of life lost (PYLL) attributable to air pollution was 69.6 and 1.85 years respectively as compared to 74.4 and 0.67 years respectively in 2013. (3) The number of the PYLL attributable to air pollution in the northern regions of China is found to be larger than that of the southern regions. The PYLL figures of the northern and southern regions in 2004 were 2.3 and 1.8 years, respectively, with a difference of 0.5 years, as compared to 1.4 and 0.7 years respectively with a difference of 0.7 years in 2013.

关键词： Air pollution     Public health     Premature deaths     Health assessment    

HTML PDF 收藏

The first avian influenza A (H7N9) viral infection in humans in Zhejiang Province, China: a death report

null

《医学前沿（英文）》 2013年 第7卷 第3期   页码 333-344 doi: 10.1007/s11684-013-0275-1

摘要：

This study reports the first death caused by a novel avian influenza A (H7N9) virus in Zhejiang Province, China. The patient had chronic hepatitis B and history of exposure to poultry. The patient initially complained of diarrhea and influenza-like symptoms on March 7 and 14 respectively. The disease progressed to severe pneumonia, sustained hypoxia, and coagulation abnormalities. The patient died on March 27 because of respiratory failure, multiple organ failure, and disseminated intravascular coagulation without oseltamivir treatment. This H7N9 virus from Zhejiang is highly similar to isolates obtained from Shanghai, Jiangsu, Anhui, etc. Analysis of hemagglutinin, neuramidinase, and matrix genes indicated that the isolates share the same avian origin, have low virulence, and are sensitive to oseltamivir, but are resistant to adamantine. Only the isolate that caused the fatality exhibited substitution of Q226I in the HA gene, which indicates a potentially enhanced human affinity. The secondary transmission rate was 1.6% (2/125). Only two health workers presented with influenza-like symptoms, and they subsequently tested negative for H7N9 RNA. In conclusion, underlying disease, late diagnosis, and untimely antiviral treatment are possible high-risk factors for infections and death caused by the low-pathogenicity avian influenza A (H7N9). Person-to-person transmission of the H7N9 virus was not detected among close contacts, but such transmission should be investigated in the future. Expanding and enhancing surveillance will help in the early discovery and diagnosis of suspected cases, which will reduce the number of severe cases and deaths.

关键词： avian influenza A (H7N9) virus     epidemiology     contacts     person-to-person transmission    

HTML PDF 收藏

Autoimmunity in acute ischemic stroke and the role of blood—brain barrier: the dark side or the light one?

Nikolay V. Tsygan, Alexandr P. Trashkov, Igor V. Litvinenko, Viktoriya A. Yakovleva, Alexandr V. Ryabtsev, Andrey G. Vasiliev, Leonid P. Churilov

《医学前沿（英文）》 2019年 第13卷 第4期   页码 420-426 doi: 10.1007/s11684-019-0688-6

摘要： This article presents a synopsis of the current data on the mechanisms of blood—brain barrier (BBB) alteration and autoimmune response in acute ischemic stroke. Most researchers confirm the relationship between the severity of immunobiochemical changes and clinical outcome of acute ischemic stroke. Ischemic stroke is accompanied by aseptic inflammation, which alters the brain tissue and exposes the co-stimulatory molecules of the immune system and the neuronal antigens. To date, BBB is not considered the border between the immune system and central nervous system, and the local immune subsystems are found within and behind the BBB. BBB disruption contributes to the leakage of brain autoantigens and induction of secondary autoimmune response to neuronal antigens and long-term inflammation. Glymphatic system function is altered and jeopardized both in hemorrhagic and ischemic stroke types. The receptors of innate immunity (toll-like receptor-2 and toll-like receptor-4) are also involved in acute ischemia—reperfusion injury. Immune response is related to the key processes of blood clotting and fibrinolysis. At the same time, the stroke-induced immune activation may promote reparation phenomena in the brain. Subsequent research on the reduction of the acute ischemic brain injury through the target regulation of the immune response is promising.

关键词： stroke     blood–brain barrier     autoimmunity     innate immunity     inflammation     cell death    

HTML PDF 收藏

假单胞菌环状脂肽Medpeptin的生物合成及其调控植物免疫的作用机制 Article

谷医林, 李俊州, 李彦, 从珅, 王静, 马毅楠, 魏海雷

《工程（英文）》 2023年 第28卷 第9期   页码 153-165 doi: 10.1016/j.eng.2023.05.016

摘要：

环状脂肽（cyclic lipopeptides, CLP）是一类由细菌产生的多功能次生代谢产物，也是一类重要的植物免疫激发子。假单胞菌环状脂肽在结构和生物活性上极其复杂和多样化。但是，目前对环状脂肽的结构及其与植物互作中的功能认识仍比较有限。本研究从地中海假单胞菌中鉴定了一种由22个氨基酸组成的新型的环状脂肽medpeptin，该脂肽由一个非核糖体肽合成酶基因簇合成并受群体感应系统调节。进一步研究发现，medpeptin可以诱导植物产生免疫反应，增强植物对病原细菌的抗性，但其自身不具备抗菌活性。利用比较转录组分析和病毒诱导的基因沉默技术解析了参与medpeptin感知植物免疫信号候选关键基因。结果显示，当沉默本氏烟中细胞壁富含亮氨酸的重复延伸蛋白NbLRX3或类受体蛋白激酶NbRLK25后，medpepatin触发的细胞死亡和对病原细菌的抗性受到严重影响，而沉默BAK1或SGT1则并未影响这一表型。本研究结果揭示了一种非典型的CLP感知机制，并为植物抗病免疫研究提供了新的借鉴。

关键词： 假单胞菌     环状脂肽     细胞死亡     富含异亮氨酸的延伸蛋白     类受体激酶    

HTML PDF 收藏

Microfluidics for cell-cell interactions: A review

Rui Li,Xuefei Lv,Xingjian Zhang,Omer Saeed,Yulin Deng

《化学科学与工程前沿（英文）》 2016年 第10卷 第1期   页码 90-98 doi: 10.1007/s11705-015-1550-2

摘要： Microfluidic chip has been applied in various biological fields owing to its low-consumption of reagents, high throughput, fluidic controllability and integrity. The well-designed microscale intermediary is also ideal for the study of cell biology. Particularly, microfluidic chip is helpful for better understanding cell-cell interactions. A general survey of recent publications would help to generalize the designs of the co-culture chips with different features. With ingenious and combinational utilization, the chips facilitate the implementation of some special co-culture models that are highly concerned in a different spatial and temporal way.

关键词： microfluidic chip     co-culture     cell-cell interactions     review    

HTML PDF 收藏

Distinct mononuclear diploid cardiac subpopulation with minimal cell–cell communications persists in

《医学前沿（英文）》   页码 939-956 doi: 10.1007/s11684-023-0987-9

摘要： A small proportion of mononuclear diploid cardiomyocytes (MNDCMs), with regeneration potential, could persist in adult mammalian heart. However, the heterogeneity of MNDCMs and changes during development remains to be illuminated. To this end, 12 645 cardiac cells were generated from embryonic day 17.5 and postnatal days 2 and 8 mice by single-cell RNA sequencing. Three cardiac developmental paths were identified: two switching to cardiomyocytes (CM) maturation with close CM–fibroblast (FB) communications and one maintaining MNDCM status with least CM–FB communications. Proliferative MNDCMs having interactions with macrophages and non-proliferative MNDCMs (non-pMNDCMs) with minimal cell–cell communications were identified in the third path. The non-pMNDCMs possessed distinct properties: the lowest mitochondrial metabolisms, the highest glycolysis, and high expression of Myl4 and Tnni1. Single-nucleus RNA sequencing and immunohistochemical staining further proved that the Myl4+Tnni1+ MNDCMs persisted in embryonic and adult hearts. These MNDCMs were mapped to the heart by integrating the spatial and single-cell transcriptomic data. In conclusion, a novel non-pMNDCM subpopulation with minimal cell–cell communications was unveiled, highlighting the importance of microenvironment contribution to CM fate during maturation. These findings could improve the understanding of MNDCM heterogeneity and cardiac development, thus providing new clues for approaches to effective cardiac regeneration.

关键词： mononuclear diploid cardiomyocytes     cell–cell communication     cardiac fibroblast     single-cell RNA sequencing     cardiac regeneration    

HTML PDF 收藏

Deubiquitinases as pivotal regulators of T cell functions

null

《医学前沿（英文）》 2018年 第12卷 第4期   页码 451-462 doi: 10.1007/s11684-018-0651-y

摘要：

T cells efficiently respond to foreign antigens to mediate immune responses against infections but are tolerant to self-tissues. Defect in T cell activation is associated with severe immune deficiencies, whereas aberrant T cell activation contributes to the pathogenesis of diverse autoimmune and inflammatory diseases. An emerging mechanism that regulates T cell activation and tolerance is ubiquitination, a reversible process of protein modification that is counter-regulated by ubiquitinating enzymes and deubiquitinases (DUBs). DUBs are isopeptidases that cleave polyubiquitin chains and remove ubiquitin from target proteins, thereby controlling the magnitude and duration of ubiquitin signaling. It is now well recognized that DUBs are crucial regulators of T cell responses and serve as potential therapeutic targets for manipulating immune responses in the treatment of immunological disorders and cancer. This review will discuss the recent progresses regarding the functions of DUBs in T cells.

关键词： deubiquitinase     ubiquitination     T cell activation     T cell differentiation     T cell tolerance    

HTML PDF 收藏

标题 作者 时间 类型 操作

Using mRNA to investigate the effect of low-pressure ultraviolet disinfection on the viability of

Chao Yang, Wenjun Sun, Xiuwei Ao

期刊论文

Orlistat induces ferroptosis-like cell death of lung cancer cells

期刊论文

Immunological effects of nano-enabled hyperthermia for solid tumors: opportunity and challenge

期刊论文

Outcomes of patients awaiting lung transplantation after the implementation of donation after brain death

期刊论文

The influence of brain death on donor liver and the potential mechanisms of protective intervention

null

期刊论文

Experimental study on the establishment and maintenance of brain death model with pigs

ZHANG Shuijun, SHI Jihua, ZHAI Wenlong, SONG Yan, CHEN Shi

期刊论文

Sudden death due to arrhythmogenic right ventricular cardiomyopathy: Two case reports

CHEN Xinshan, ZHANG Yigu, RAO Guangxun, HUANG Guangzhao

期刊论文

Protein aggregation in association with delayed neuronal death in rat model of brain ischemia

GE Pengfei, FU Shuanglin, LI Wenchen, WANG Chonghao, ZHOU Chuibing, LUO Yinan, LUO Tianfei

期刊论文

Assessing the premature death due to ambient particulate matter in China’s urban areas from 2004 to 2013

Guoxia MA, Jinnan WANG, Fang YU, Xiaomin GUO, Yanshen ZHANG, Chao LI

期刊论文

The first avian influenza A (H7N9) viral infection in humans in Zhejiang Province, China: a death report

null

期刊论文

Autoimmunity in acute ischemic stroke and the role of blood—brain barrier: the dark side or the light one?

Nikolay V. Tsygan, Alexandr P. Trashkov, Igor V. Litvinenko, Viktoriya A. Yakovleva, Alexandr V. Ryabtsev, Andrey G. Vasiliev, Leonid P. Churilov

期刊论文

假单胞菌环状脂肽Medpeptin的生物合成及其调控植物免疫的作用机制

谷医林, 李俊州, 李彦, 从珅, 王静, 马毅楠, 魏海雷

期刊论文

Microfluidics for cell-cell interactions: A review

Rui Li,Xuefei Lv,Xingjian Zhang,Omer Saeed,Yulin Deng

期刊论文

Distinct mononuclear diploid cardiac subpopulation with minimal cell–cell communications persists in

期刊论文

Deubiquitinases as pivotal regulators of T cell functions

null

期刊论文